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KMID : 0613820020120060708
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Journal of Life Science
2002 Volume.12 No. 6 p.708 ~ p.714
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Leaf Blight of Perilla Caused by Alternaria alternata
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Cha Wea-Jin
Kim Choul-Soung
Song Ju-Hee
Kim Hyun-Ju
Yi Young-Byung
Moon Byung-Ju
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Abstract
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A new leaf blight was found on the perilla leaves at the major perilla-cultivating areas such as Kangdong in Busan and Miryang in Kyungnam province. Symptoms of the disease initially appeared on the edge of perilla leaves showing black necrosis and drying, and the infected leaves were finally fell down. The SD1 isolate showing strong pathogenicity and forming abundant conidial spores on the diseased lesions was isolated. Among the tested media, mycelial growth was abundant on PDA (Potato Dextrose Agar) medium at 25℃ under dark condition, but conidial formation was greater on V8A (V-8 juice Agar) medium than that on PDA medium. Optimal temperatures for mycelial growth and conidial formation on PDA medium were respectively 25℃ and 30℃. The rate of conidial germination and the elongation of germ tube were more effective in 10% tomato juice than those in PDB (Potato Dextrose Broth) and sterile water. In 10% tomato juice, the rate of conidial germination and the length of germ tube were 100% after incubation for 24hr and 535.2㎛ after incubation for 36hr, respectively. According to the result of pathogenicity, it revealed that conidial suspention with 10% tomato juice was the most effective for pathogenicity test showing as 100% of disease incidence, and the symptoms caused by artificial inoculum were same as those of naturally infected perilla. In this study, the SD1 isolate according to the results of morphological characteristics, the incubation characteristics and pathogenicity was firstly identified A. alternata, and named as leaf blight of perilla.
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KEYWORD
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Alternaria alternata, Leaf blight of perilla, Pathogenicity, Tomato juice
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